Liquid chromatography apparatus - List of Manufacturers, Suppliers, Companies and Products

Transfer of the analysis method for chlorhexidine impurities.

We will explain the key points for successfully transferring HPLC analysis methods using the results of rigorous analytical comparisons!

Transferring liquid chromatography (LC) methods between instruments is a challenging task that many analytical laboratories frequently face. The success of this method transfer depends on many factors, making it important to reduce the differences between the systems being transferred and the robustness of the method being transferred. This application note presents a smooth transfer from the Thermo Scientific UltiMate 3000 HPLC system to the Thermo Scientific Vanquish Core HPLC system using an HPLC method from the European Pharmacopoeia.

Company：サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K.
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Composed of biocompatible materials: Universal binary UHPLC

Used in biopharmaceuticals (such as critical quality attributes) and other applications utilizing high salt concentration and high pH conditions.

The "1290 Infinity II Bio LC System" is a universal binary UHPLC composed of biocompatible materials. It is bio-compatible and ensures the integrity of biomolecules and the robustness of the system. Based on the proven 1290 Infinity II LC technology, method transfer can be easily implemented. Transferring from conventional equipment is also simple, saving time and effort in training, and it exhibits very high separation capability and resolution at pressures of up to 130 MPa, minimizing dispersion. 【Features】 ■ Ensures the integrity of biomolecules while minimizing unwanted surface interactions ■ Maximizes chromatographic resolution and minimizes dispersion ■ Improved flexibility and robustness, increasing equipment uptime ■ Saves effort in training ■ Can accommodate up to 6,144 samples, achieving excellent sample capacity *For more details, please refer to the PDF document or feel free to contact us.

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[Case Study] Analysis of Tocopherols α, β, γ, and δ using 2D-LC

Vitamin E (alpha, beta, gamma, delta tocopherols) in vegetable oils can be separated and quantified without the influence of interfering components using single-loop heart-cut 2D-LC.

This document introduces a method for the high-precision separation and quantification of four types of tocopherols contained in vegetable oils using the single-loop heart-cut 2D-LC method. Under the separation conditions of a reverse-phase system using a general ODS column, the separation of β-tocopherol and γ-tocopherol is difficult; however, the 2D-LC method allows for easy separation. In the first dimension, tocopherols and interfering components in the sample are separated, and in the second dimension, the four types of tocopherols (α, β, γ, δ) are separated. The repeatability of the standard samples achieved a high reproducibility with RSD < 0.1%. Additionally, measurements conducted on multiple vegetable oils showed that quantification was possible without the influence of interfering peaks. The single-loop heart-cut 2D-LC method is the simplest technique among 2D-LC methods, making it applicable for routine analysis and a useful tool for food analysis involving samples with a high content of matrix components. 【Features】 ■ High-precision separation achieved with the single-loop heart-cut 2D-LC method ■ Separation of β-tocopherol and γ-tocopherol, which is difficult in reverse-phase systems ■ Effective separation of interfering substances in food ■ High reproducibility with repeatability of RSD < 0.1%

Company：サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K.
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What are the types of separation modes in HPLC? Free explanatory materials available.

What are the characteristics of each separation mode in HPLC? A comprehensive and easy-to-understand explanation of the basics of HPLC! Free basic knowledge materials are currently available.

In chromatography, the differences in the affinity of substances for the stationary phase and the mobile phase are utilized. The separation modes commonly used in HPLC can be broadly classified into adsorption, partition, and size exclusion based on the type of affinity. The main separation modes include "adsorption," "partition," "hydrophilic interaction," "ion exchange," and "size exclusion." This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is distribution chromatography? Free explanatory materials are currently available.

What is one of the separation modes of HPLC, 'Partition Chromatography'? A thorough explanation of the differences between normal phase and reverse phase! Free basic knowledge materials available!

Partition chromatography separates compounds by utilizing the differences in the distribution equilibrium of the analyte with respect to the stationary phase. The stationary phase typically consists of a chemically bonded packing material known as a bonded phase. There are two types of partition chromatography: normal phase and reverse phase. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is a valuable resource for those learning HPLC for the first time, those looking to review from the basics, and those seeking materials for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is reverse phase liquid chromatography? Free explanatory materials available.

What is reverse phase liquid chromatography? A thorough and easy-to-understand explanation of its differences from normal phase, with free basic knowledge materials available!

Reverse phase liquid chromatography uses a packing material with a low-polarity chemically bonded phase as the stationary phase and a high-polarity solvent as the mobile phase. High-polarity compounds are less retained by the stationary phase and elute quickly, while low-polarity compounds elute more slowly. Octadecylsilyl silica gel (ODS), which incorporates octadecyl groups (C18) into silica gel, is a widely used packing material in HPLC. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to HPLC, want to review from the basics, or are looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is ion exchange chromatography? Free explanatory materials are currently available.

What is ion exchange chromatography? A comprehensive explanation based on a comparison table by separation mode! Free basic knowledge materials available.

Ion exchange chromatography separates ionic compounds using a stationary phase that incorporates ion exchange groups (such as sulfonic groups for cation exchange or quaternary ammonium groups for anion exchange) and a mobile phase that consists of an aqueous solution containing salts, such as a buffer. It is used for the separation of amino acids, organic acids, and proteins. By downloading the materials, you can view examples of protein analysis using cation exchange columns. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who wish to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is size exclusion chromatography? Free explanatory materials are currently available.

What is size exclusion chromatography? A comprehensive explanation based on a comparison table of separation modes! Free basic knowledge materials available!

Size exclusion chromatography separates compounds based on differences in molecular size of the analytes in the mobile phase, rather than interactions between the stationary phase and the analytes. Molecules smaller than the pore size of the packing material pass slowly through the column, while molecules larger than the pore size pass quickly through the column. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is a valuable resource for those learning HPLC for the first time, those who want to review the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is the difference between isocratic elution and gradient elution? Presentation of explanatory materials available.

Understand how to choose between isocratic elution and gradient elution! A thorough and easy-to-understand explanation of their features, differences, and suitable scenarios! Free basic knowledge materials available!

In HPLC, there are isocratic elution, which maintains the composition of the mobile phase for separation, and gradient elution, which varies the composition of the mobile phase for separation. Generally, isocratic elution is used for analyzing multiple components where there is little difference in retention time for each component, while gradient elution is used for analyzing multiple components with significant differences in retention time. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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[Case Study] Simultaneous Analysis of Vitamins A, D, and E using 2D-LC

Simplifying the preprocessing with the single loop heart cut 2D-LC method significantly reduces analysis time. It enables the highly sensitive detection of vitamins A, D, E, and their isomers.

This technical document introduces a rapid and quantitative analytical method for the simultaneous detection of seven types of vitamins (vitamin A, vitamin D2, vitamin D3, and four types of vitamin E isomers) in infant formula using a conventional HPLC system with a diode array detector and a fluorescence detector, following offline solid-phase extraction (SPE) and two-dimensional liquid chromatography (2D-LC) analysis. By changing the traditional liquid-liquid extraction method to solid-phase extraction, the time required for sample preparation was reduced from 3 hours to 1 hour. Additionally, by simplifying the sample preparation and optimizing the separation conditions, the influence of the matrix was reduced, allowing for the detection of target substances with higher sensitivity. ■ Simplified sample preparation through two-dimensional liquid chromatography (2D-LC) analysis ■ Simultaneous separation analysis of vitamins A, D, E, and their isomers ■ Developed a method with higher recovery rates and sensitivity than liquid-liquid extraction ■ Simplified the preprocessing process and reduced analysis time by 2.5 times ■ Reduced the influence of the matrix and provided reliable data

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Understand in 3 minutes! What is the configuration of HPLC equipment? Free explanatory materials available.

What is the configuration of HPLC (High-Performance Liquid Chromatography) equipment? A thorough and easy-to-understand explanation of the basics of HPLC! Free basic knowledge materials available!

High performance liquid chromatography (HPLC) is a type of liquid chromatography that achieves high separation efficiency by pressurizing the mobile phase liquid and using high-performance columns. It is utilized in various fields of chemical analysis. The HPLC system consists of a pump that delivers the mobile phase, an injector for sample injection, a column that separates the components in the sample, a column thermostat that maintains a constant column temperature, and a detector that identifies the separated components. This document provides a clear summary of the fundamentals of liquid chromatography (LC) separation, detectors, and principles. It is a valuable resource for those who are learning HPLC for the first time, those who wish to review from the basics, and those looking for educational materials for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is adsorption chromatography? Free explanatory materials are being offered.

What is "Adsorption Chromatography," one of the separation modes of HPLC? A thorough explanation based on a comparison table of different separation modes! Free basic knowledge materials available!

Adsorption chromatography separates compounds by utilizing the difference in adsorption equilibrium between the adsorption sites of the stationary phase (packing material) and the analytes. Silica gel and alumina are used as the stationary phase, while hexane and dichloromethane are used as the mobile phase. It is effective for the separation of isomers. This document provides a clear summary of the basics of liquid chromatography (LC), detectors, and principles. It is a valuable resource for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is normal phase liquid chromatography? Free explanatory materials are currently available.

What is normal phase liquid chromatography? A thorough and easy-to-understand explanation of the differences from reverse phase! Free basic knowledge materials available.

Normal-phase liquid chromatography uses a stationary phase consisting of a highly polar chemically bonded phase and a mobile phase of low-polarity solvents. Because highly polar compounds are strongly retained by the stationary phase, low-polarity compounds elute quickly. It is used for the analysis of lipophilic compounds and the separation of organic compounds by groups. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is ion chromatography? Free explanatory materials are currently available.

What is ion chromatography? A comprehensive and easy-to-understand explanation of other methods of partition chromatography! Free basic knowledge materials are available!

Ion-pair chromatography involves adding ionic compounds known as ion-pair reagents (such as alkyl sulfonates and quaternary ammonium salts) to the mobile phase to separate ionic compounds that are not retained in RPC using the stationary phase of RPC. Ion-pair reagents are used that have charges opposite to those of the analytes; for anionic compounds, quaternary ammonium salts are used, and for cationic compounds, alkyl sulfonates are used. This document provides a clear summary of the basics of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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What is hydrophilic interaction chromatography? Free explanatory materials are being offered.

"What is hydrophilic interaction chromatography?" A comprehensive explanation based on a comparison table by separation mode! Free basic knowledge materials available!

Hydrophilic interaction chromatography (HILIC) uses a stationary phase consisting of high-polarity bonded phases such as amino groups, with packing materials or silica gel, and a mobile phase of a mixed solution of water (buffer) and acetonitrile. It is suitable for the separation of compounds with weak retention (high polarity) in RPC. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

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Liquid chromatography apparatus - List of Manufacturers, Suppliers, Companies and Products

Liquid chromatography apparatus Product List